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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is noticed in many tumors, which leads to chromosome instability, and thus may possibly add to tumorigenesis. This protein was found to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical purpose in selling mobile-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, are actually explained. Two added splice variants were being documented but in-depth nucleotide sequence information is not really nonetheless out there.
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Blocking peptides are peptides that bind exclusively to the goal antibody and block antibody binding. These peptide commonly consists of the epitope identified from the antibody.
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This information outlines further probable optimization of mobile and immunoassay parameters to make certain the best possible success are...
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This guideline outlines further possible optimization of cellular and immunoassay parameters to make sure the best possible benefits are...
Antibodies sure to the blocking peptide no longer bind for the epitope within the goal protein. By evaluating the staining from your blocked antibody as opposed to the antibody by yourself, you can see which staining is distinct.
Concentrate on Information Cyclin E belongs for the hugely conserved cyclin household, whose associates are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins operate as regulators of CDK kinases. Unique cyclins show distinctive expression and degradation styles which add to your temporal coordination of each and every mitotic event. Cyclin E varieties a fancy with and functions for a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates at the G1-S section boundary and it is degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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